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KMID : 0379520040200040365
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한국독성학회지
2004 Volume.20 No. 4 p.365 ~ p.374
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Safety Evaluation of Tobacco Substitute (Herbrette); Inhalation Toxicity, Mutagenicity and Immunotoxicity
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Song Kyung-Seuk
Park Gun-Ho
Yoo Gi-Yong
Song Sung-Ok
Kim Hyun-Woo
Kim J.-S.
Park Jin-Hong
Eu Gook-Jong
Hur Jin
Cho Hyun-Sun
Hwang Soon-Kyung
Chang Seung-Hee
Tehrani Arash Minai
Yu Keong-Nam
Chae Chan-Hee
Cho Myung-Haeng
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Abstract
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Inhalation toxicity, mutagenicity, and immunotoxicity tests were performed using a smoke generation system to investigate the safety of Herbrette, a tobacco substitute made with the leaves of Perilla frutescens. ICR mice were exposed to nicotine-free Herbrette smoke with concentrations of 0 (control), 4.08 pm 1.32 mg/m^3 (low dose), 7.72 pm 2.14 mg/m^3 (medium dose) and 12.83 pm 1.69 mg/m^3 (high dose) total particulate matters (TPM) for 4 weeks. When compared to the control group, the body weights, organ weights in the exposed groups did not show any significant differences. However, certain change of several serum chemical data and biochemical parameters were observed, however, the changes were within normal physiological ranges. Moreover, no changes in organ weight, and no gross/microscopic changes were observed between the exposed and control groups. Salmonella typhimurium reverse mutation, in vivo chromosomal aberration and micronucleus assays revealed that Herbrette did not induce mutagenicity. Upon evaluation of peripheral cellular immunity of mice through in vitro lymphocyte proliferation assay, no significant difference was observed in mean stimulation index between the exposed and control groups. Taken together, our results strongly suggest that Herbrette may not cause toxicity on mice under current condition.
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KEYWORD
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Tobacco substitute, Herbrette, Inhalation toxicity, Mutagenicity, Immunotoxicity
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